KeyTec® TR-FRET Phospho-AKT (S473) kit
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CAT.: A1060352D
The KeyTec® TR-FRET AKT (S473) Phosphorylation Assay Kit is designed to measure the phosphorylation level of AKT at serine 473 (S473) in intracellular lysates. This kit employs a sandwich immunoassay format developed with KeyTec® TR-FRET technology, offering advantages such as simple and rapid operation, high specificity, high sensitivity, and excellent reproducibility. As illustrated in the schematic diagram, a pair of specific antibodies are conjugated to the fluorescence energy donor (Solar Eu1) and acceptor (LA2), respectively. Upon phosphorylation at the S473 site of AKT, both antibodies simultaneously bind to distinct epitopes on the AKT protein. Under external light excitation, energy resonance transfer occurs between the donor and acceptor. The phosphorylation level of AKT at S473 is then determined by measuring the signal intensity at the specific wavelength of 665 nm.
Stimulate the phosphorylation of AKT at Ser473 in NIH/3T3-L1 cells
NIH/3T3-L1 cell lysate titration (QC test)
| CAT. | Description | Size |
| A1060352D | KeyTec® TR-FRET Phospho-AKT (S473) kit | 100 tests |
|
• 1 vial 5 µL/vial Phospho-AKT(S473) Solar Eu & LA Abs mixture • 1 vial 40 µL/vial Positive control lysate • 1 vial 50 µL/vial Phosphatase Inhibitor Cocktail • 1 vial 1 mL/vial KeyTec® TR-FRET Phospho and Total protein Lysis Buffer #5 • 1 vial 500 µL/vial KeyTec® TR-FRET Phospho and Total protein Detection Buffer #5 |
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| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
The KeyTec® TR-FRET AKT (S473) Phosphorylation Assay Kit is designed to measure the phosphorylation level of AKT at serine 473 (S473) in intracellular lysates. This kit employs a sandwich immunoassay format developed with KeyTec® TR-FRET technology, offering advantages such as simple and rapid operation, high specificity, high sensitivity, and excellent reproducibility. As illustrated in the schematic diagram, a pair of specific antibodies are conjugated to the fluorescence energy donor (Solar Eu1) and acceptor (LA2), respectively. Upon phosphorylation at the S473 site of AKT, both antibodies simultaneously bind to distinct epitopes on the AKT protein. Under external light excitation, energy resonance transfer occurs between the donor and acceptor. The phosphorylation level of AKT at S473 is then determined by measuring the signal intensity at the specific wavelength of 665 nm.
Stimulate the phosphorylation of AKT at Ser473 in NIH/3T3-L1 cells
NIH/3T3-L1 cell lysate titration (QC test)
| CAT. | Description | Size |
| A1060352S | KeyTec® TR-FRET Phospho-AKT (S473) kit | 500 tests |
|
• 1 vial 25 µL/vial Phospho-AKT(S473) Solar Eu & LA Abs mixture • 1 vial 100 µL/vial Positive control lysate • 1 vial 400 µL/vial Phosphatase Inhibitor Cocktail • 1 bottle 8 mL/bottle KeyTec® TR-FRET Phospho and Total protein Lysis Buffer #5 • 1 bottle 2.5 mL/bottle KeyTec® TR-FRET Phospho and Total protein Detection Buffer #5 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
The KeyTec® TR-FRET AKT (S473) Phosphorylation Assay Kit is designed to measure the phosphorylation level of AKT at serine 473 (S473) in intracellular lysates. This kit employs a sandwich immunoassay format developed with KeyTec® TR-FRET technology, offering advantages such as simple and rapid operation, high specificity, high sensitivity, and excellent reproducibility. As illustrated in the schematic diagram, a pair of specific antibodies are conjugated to the fluorescence energy donor (Solar Eu1) and acceptor (LA2), respectively. Upon phosphorylation at the S473 site of AKT, both antibodies simultaneously bind to distinct epitopes on the AKT protein. Under external light excitation, energy resonance transfer occurs between the donor and acceptor. The phosphorylation level of AKT at S473 is then determined by measuring the signal intensity at the specific wavelength of 665 nm.
Stimulate the phosphorylation of AKT at Ser473 in NIH/3T3-L1 cells
NIH/3T3-L1 cell lysate titration (QC test)
| CAT. | Description | Size |
| A1060352L | KeyTec® TR-FRET Phospho-AKT (S473) kit | 5,000 tests |
|
• 1 vial 250 µL/vial Phospho-AKT(S473) Solar Eu & LA Abs mixture • 2 vials 100 µL/vial Positive control lysate • 1 bottle 3 mL/bottle Phosphatase Inhibitor Cocktail • 1 bottle 60 mL/bottle KeyTec® TR-FRET Phospho and Total protein Lysis Buffer #5 • 1 bottle 25 mL/bottle KeyTec® TR-FRET Phospho and Total protein Detection Buffer #5 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
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